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Angiocidin

A Novel Therapeutic Recombinant Protein for AML

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Angiocidin

  • Overview
  • Target
  • Indication
  • Mechanism of Action
  • Status
  • Data
Drug Name Angiocidin
Description

Angiocidin a 41 kDa recombinant human protein that binds to the TNF superfamily death receptor 6 (DR6) to drive differentiation of leukemic cells and activate the host immune system to fight against acute myeloid leukemia (AML).

As a new therapeutic:
• Angiocidin increases survival rate, reduces weight loss, and suppresses
    tumor growth in mouse AML model.
• Angiocidin exhibits no observed toxicity.
• Angiocidin can work synergistically with chemotherapeutics to increase
    tumor clearance.

Target DR6
Drug Modality Recombinant protein
Indication Acute myeloid leukemia
Product Category Immunotherapy
Mechanism of Action Angiocidin, binding to DR6, induces differentiation of leukemic cells and activates host immune system to fight against cancer.
Status Preclinical
Patent US patents have been issued for Angiocidin to treat leukemia and solid tumors.

Collaboration Opportunity

Protheragen Inc. is actively seeking partnership to further develop Angiocidin. Potential collaboration can be strategic alliance, licensing, or marketing agreement.

We look forward to hearing from you.

Death Receptor 6 (DR6)

Introduction

This gene encodes a member of the tumor necrosis factor receptor superfamily. The encoded protein activates nuclear factor kappa-B and mitogen-activated protein kinase 8 (also called c-Jun N-terminal kinase 1) and induces cell apoptosis. Through its death domain, the encoded receptor interacts with tumor necrosis factor receptor type 1-associated death domain (TRADD) protein, which is known to mediate signal transduction of tumor necrosis factor receptors. Knockout studies in mice suggest that this gene plays a role in T-helper cell activation, and may be involved in inflammation and immune regulation.

The deduced 655-amino acid protein has an N-terminal signal sequence followed by 4 TNFR-like cysteine-rich motifs, and a transmembrane domain followed by a cytoplasmic portion containing a 135-amino acid death domain and a 150-residue tail. The death domain of DR6 is 27% identical to that of TNFR1.

Approved Name TNF receptor superfamily member 21
Official Symbol TNFRSF21
Gene Type Protein coding
Synonyms TNFRSF21, BM-018, CD358, DR6, tumor necrosis factor receptor superfamily member 21, TNF receptor superfamily member 21
Ensembl ENSG00000146072
Gene ID 27242
mRNA Refseq NM_014452
Protein Refseq NP_055267
OMIM 605732
UniProt ID O75509
Chromosome Location 6p12.3

Clinical Resources

Gene Function Within the tumor necrosis factor receptor superfamily, a subgroup, or the death receptor, contains a cytoplasmic death domain. Activation of these receptors leads to the engagement of components of the cell death pathway, including the adaptor molecule TRADD and subsequently the FADD-caspase-8 pathway, which in turn activates the nuclear factor kappa-B pathway. TNFRSF21 belongs to the death receptor subfamily of TNFRs.
Pathway Rho protein geranylgeranylation and nuclear factor kappa B signaling pathway

Acute Myeloid Leukemia (AML)

Leukemia is heterogeneous class of a malignant diseases of the bone marrow and blood that are characterized by the uncontrolled proliferation of undifferentiated hematopoietic cells. Leukemia is classified as either myelogenous or lymphocytic, according to the type of cell involved. Leukemia is furthermore classified as either chronic or acute, based on the clinical presentation and course.

Acute myeloid (or myelogenous) leukemia (AML) is the most common malignant myeloid disorder in adults, and is associated with the lowest survival rate, although survival has improved steadily in recent decades. It affects more men than women, with a median age of 70 years at diagnosis. In adult patients, the highest rates of AML are seen in North America, Europe, and Oceania, while adult AML is rarer in Asia and Latin America.

The hallmarks of AML are an abnormal proliferation of myeloid progenitor cells in bone marrow, reduced rate of self-destruction and arrest in cellular differentiation. When the blast cells lose their ability to differentiate in a normal fashion and to respond to normal regulators of cell proliferation, the result is frequent infections, bleeding and organ infiltration. The leukemic cells are endowed with an abnormal survival advantage with respect to normal healthy cells, such that the bone marrow and peripheral blood become increasingly populated by immature blast cells that edge out normal blood cells. Bone marrow failure is the most common cause of death in patients with AML.

The main treatment for most types of AML is chemotherapy, sometimes along with a targeted therapy drug. This might be followed by a stem cell transplant. Surgery and radiation therapy are not major treatments for AML, but may be used in special circumstances.

Inducing Monocyte Differentiation and Activating the Host Immune System

Highly expressed in monocytes and leukemia cell lines, DR6 represents an attractive novel target for AML research. Binding to DR6, Angiocidin drives the NF-kB pathway, induces irreversible differentiation of monocytes into an activated macrophage phenotype, and activated macrophages release cytokines such as IL-2 that activate T-cells. In addition, Angiocidin induces irreversible terminal differentiation in AML cells, reducing the continued progression of the disease and potentiating chemotherapy.

As a new therapeutic for AML, Angiocidin also stimulates AML cell differentiation, reducing the spread of disease and potentiating chemotherapy.

Status of Angiocidin

A series of in vivo and in vitro experiments were completed, such as immunoregulatory activity tests and AML xenograft mouse model tests. US patents of Angiocidin treating solid tumors and leukemia have been issued.


Effect of Angiocidin on the Monocytic Cell Line THP-1

Angiocidin-treated THP-1 cells underwent dramatic morphological changes when treated with as little as 1.0 μg/ml Angiocidin for 24 h. THP-1 cells became adherent and spread on tissue culture plastic as well as other matrix proteins such as collagen.


Effect of Angiocidin on Lymphocyte

Normal human PBMCs were treated with 1.0 μg/ml of Angiocidin for 6 h and analyzed by flow cytometry. CD4+ and CD8+ lymphocytes were analyzed for the activation markers HLA-DR and CD69.


Effect of Angiocidin on A Xenotransplanted Primary Human AML Sample Engrafted in NSG Mice

Human leukemic cells, with positive CD45 and CD33 human cell surface markers, were identified and counted by flow cytometry. Angiocidin monotherapy and Angiocidin + Ara C (cytosine arabinoside) combination therapy reduced human AML burden (CD45+/CD33+ cells) in the bone marrow of NSG mice xenotransplanted with primary human cells.

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